Journal: Heliyon

Article Title: Modified Zhenwu Tang delays chronic renal failure progression by modulating oxidative stress and hypoxic responses in renal proximal tubular epithelial cells.

doi: 10.1016/j.heliyon.2024.e31265

Figure Lengend Snippet: Fig. 3. Adenine-induced oxidative stress, activation of the HIF pathway, and increased production of inflammatory factors and tubulointerstitial collagen fibers in rat proximal renal tubules, with MZWT mitigating these effects. (a) Immunohistochemical detection of SOD1, MDA, HIF-1α, COL1A1, IL-1β, TNF-α in rat renal tissues (IHC × 400). (b) Quantitative analysis of the positive expression areas for each marker. (N: normal group, M: model group, MZWT: Modified Zhenwu Tang group, Lotensin: benazepril hydrochloride group. ##P < 0.01, #P < 0.05 compared with the N group; **P < 0.01, *P < 0.05 compared with the M group; NS indicates no statistical significance).

Article Snippet: Zhang et al. Heliyon 10 (2024) e31265 temperature, and incubated with α-SMA and COL1A1 antibodies (1:100, boster, BM0002, bs-10423).

Techniques: Activation Assay, Immunohistochemical staining, Immunohistochemistry, Expressing, Marker, Modification

Journal: Heliyon

Article Title: Modified Zhenwu Tang delays chronic renal failure progression by modulating oxidative stress and hypoxic responses in renal proximal tubular epithelial cells.

doi: 10.1016/j.heliyon.2024.e31265

Figure Lengend Snippet: Fig. 12. Expression of fibrotic markers α-SMA and COL1A1 in HK-2 cells under hypoxic conditions and LPS treatment, showing reduction by MZWT (IF × 200). Details of expression: (a) α-SMA, (b) Relative intensity of α-SMA, (c) COL1A1, (d) Relative intensity of COL1A1. Groups: (A: Normal group, B: Model group, C: Blank serum group, D: Drug serum group, E: FM19G11 group, F: NAC group). Statistical significance: ##P < 0.01, #P < 0.05 compared with the A group; **P < 0.01, *P < 0.05 compared with the B group; NS indicates no statistical significance.

Article Snippet: Zhang et al. Heliyon 10 (2024) e31265 temperature, and incubated with α-SMA and COL1A1 antibodies (1:100, boster, BM0002, bs-10423).

Techniques: Expressing

Journal: International Journal of Molecular Sciences

Article Title: Differentiation Effects of Platelet-Rich Plasma Concentrations on Synovial Fluid Mesenchymal Stem Cells from Pigs Cultivated in Alginate Complex Hydrogel

doi: 10.3390/ijms160818507

Figure Lengend Snippet: Evaluation of differentiation potential of SF-MSCs and bone marrow (BM)-MSCs by gene expressions. Gene expression profiles of SF-MSCs and BM-MSCs after osteogenic, chondrogenic, and adipogenic induction. Among them, type I collagen, osteocalcin, and osteopontin were for osteogenesis; type II collagen and aggrecan were for chondrogenesis; and peroxisome proliferator activated receptor γ 2 (PPAγ2) and adipocyte protein 2 (aP2) were for adipogenesis, respectively. Three SF-MSCs and BM-MSCs samples were performed for this experiment.

Article Snippet: Following permeabilization with 0.2% Triton X-100 (USB Corp., Cleveland, OH, USA) and blocking solution treatment (5% non-fat milk in PBS with 0.1% Triton X-100) for 30 min at room temperature, the slides were incubated with mouse anti-porcine monoclonal primary antibodies of type I collagen, type II collagen, and PPAγ2 (Merck Millipore, Darmstadt, Germany), applied for 1.5 h at room temperature.

Techniques: Expressing

Journal: International Journal of Molecular Sciences

Article Title: Differentiation Effects of Platelet-Rich Plasma Concentrations on Synovial Fluid Mesenchymal Stem Cells from Pigs Cultivated in Alginate Complex Hydrogel

doi: 10.3390/ijms160818507

Figure Lengend Snippet: Evaluation of differentiation potential of SF-MSCs by histological staining and immunofluorescence staining. Histological staining ( A ) and immunofluorescence staining ( B ) of SF-MSCs after osteogenic (Alizarin red S stain and Type I collagen), chondrogenic (Alcain blue stain and Type II collagen), and adipogenic (Oil red O stain and PPAγ2) induction. Green: extracellular matrix and blue: cell nuclei.

Article Snippet: Following permeabilization with 0.2% Triton X-100 (USB Corp., Cleveland, OH, USA) and blocking solution treatment (5% non-fat milk in PBS with 0.1% Triton X-100) for 30 min at room temperature, the slides were incubated with mouse anti-porcine monoclonal primary antibodies of type I collagen, type II collagen, and PPAγ2 (Merck Millipore, Darmstadt, Germany), applied for 1.5 h at room temperature.

Techniques: Staining, Immunofluorescence

Journal: International Journal of Molecular Sciences

Article Title: Differentiation Effects of Platelet-Rich Plasma Concentrations on Synovial Fluid Mesenchymal Stem Cells from Pigs Cultivated in Alginate Complex Hydrogel

doi: 10.3390/ijms160818507

Figure Lengend Snippet: Sequences of primers used in real-time PCR.

Article Snippet: Following permeabilization with 0.2% Triton X-100 (USB Corp., Cleveland, OH, USA) and blocking solution treatment (5% non-fat milk in PBS with 0.1% Triton X-100) for 30 min at room temperature, the slides were incubated with mouse anti-porcine monoclonal primary antibodies of type I collagen, type II collagen, and PPAγ2 (Merck Millipore, Darmstadt, Germany), applied for 1.5 h at room temperature.

Techniques: